Figure 8.

K⁺ is exchanged between reticulate bodies. (A) HeLa cells were infected with C. trachomatis LGV2. At 24 hpi cells were labelled with APG-2 and DRAQ-5 and then imaged. APG-2 was photobleached as indicated on the upper panels in some reticulate bodies (RBs). Circles show the region of interest used for the analysis of the intensity depending on the frame (lower panels: 1.509 s/frame) presented on the lower panels. Dark blue: unbleached RBs and light blue: bleached RBs. Scale bar: 5 µm. (B) Shows schematics of the proposed K⁺ flux (pink arrows), between the host cytoplasm (white) the inclusion lumen (light blue) and the bacteria (dark blue).