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. 2021 Jan 17;26(2):465. doi: 10.3390/molecules26020465

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Determination of the stages of cell cycle arrest following treatment with CBC + THC (17.2 + 2.8 μg/mL, respectively) or CBD (15 μg/mL) on the T24 cell line for 24 h. Mitomycin-C (MMC, 8 µg/mL) served as a positive control. Methanol (control) treatment served as a solvent (vehicle) control. Error bars indicate ± SE (n = 3). Levels with different letters of the same font are significantly different from all combinations of pairs according to Tukey–Kramer honest significant difference (HSD; p ≤ 0.05). * indicates significantly different mean from the control based on Student t-test (p ≤ 0.05). (b) Proportion of viable, apoptotic, or necrotic cells following treatment with CBC+THC (17.2 + 2.8 μg/mL, respectively) or CBD (15 μg/mL) on T24 cell line for 48 h. Mitomycin-C (MMC, 8 µg/mL) served as a positive control. Methanol (control) treatment served as solvent (vehicle) control. Error bars indicate ± SE (n = 3 for treatments, n = 4 for the control). Levels with different letters are significantly different from all combinations of pairs according to Tukey-Kramer honest significant difference (HSD; p ≤ 0.05).