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. 2021 Jan 16;9(1):56. doi: 10.3390/vaccines9010056

Figure 3.

Figure 3

Upon challenge with GM3-αGC liposomes CD169+ macrophages are indispensable for the activation of OVA-specific CD8+ T cells but not for activation of NKT, NK and B cells. The activation of (A) NKT and (B) NK cells in CD169-DTR animals treated with DT or left untreated, analyzed at 2 h p.i. with liposomes illustrated by the frequency of the IFNγ-producing cells and gMFI, determined by flow cytometry. (C,D, left panel) The activation of antigen-specific T and B cells in CD169-DTR animals treated with DT or left untreated, analyzed at day 7 p.i. with liposomes illustrated by frequency of SIINFEKL+ CD8+ T cells, IFNγ production by CD8+ and CD4+ T cells and frequency of OVA+ germinal center B cells, determined by flow cytometry. (D, middle and right panels) The activation of B cells in CD169-DTR animals treated with DT (closed symbols) or left untreated (open symbols) on day 7 p.i. with liposomes illustrated by serum total anti-OVA Ig titer, determined by ELISA. The data are the mean ± SEM (A–D) or geometric mean + 95% CI (D, right panel) from one experiment with 3–5 mice per group. Each symbol represents one mouse (one-way ANOVA with Tukey’s test: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).