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. 2021 Jan 16;9(1):56. doi: 10.3390/vaccines9010056

Figure 4.

Figure 4

Vaccination with GM3-αGC liposomes triggers NKT, NK cell and CD8+ T cell responses that are dependent on cDC1.Activation of (A) NKT and (B) NK cells in WT and Batf3KO animals analyzed at 2 h p.i. with liposomes, illustrated by frequency of IFNγ-producing cells and gMFI, determined by flow cytometry. (C,D, left panel) Activation of antigen-specific T and B cells in WT and Batf3KO animals analyzed at day 7 p.i. with liposomes illustrated by the frequency of SIINFEKL+ CD8+ T cells, IFNγ production by CD8+ and CD4+ T cells and the frequency of OVA+ germinal center B cells, determined by flow cytometry (D, middle and right panels). Activation of B cells in WT (close symbols) and Batf3KO (open symbols) animals on day 7 p.i. with liposomes illustrated by serum total anti-OVA Ig titer, determined by ELISA. The data are the mean ± SEM (A–D) or geometric mean + 95% CI (D, right panel) from one experiment with 3–5 mice per group. Each symbol represents one mouse (one-way ANOVA with Tukey’s test: * p < 0.05, ** p < 0.01, **** p < 0.0001).