Table 1.
The antibodies and fluorescently labelled reagents used for flow cytometry.
| Antigen/reagent | Clone | Fluorochrome | Company |
|---|---|---|---|
| NK1.1 | PK136 | BV711 | Biolegend |
| CD25 | PC61 | BV650 | Biolegend |
| CD69 | H1.2F3 | AF700 | Biologend |
| CD3 | KT-3 | Alexa Fluor 488 | In-house made |
| CD4 | GK1.5 | BV510 | Biolegend |
| CD8 | 53-6.7 | PerCP-Cy5.5 | Biolegend |
| IL-4 | 11B11 | BV421 | Biolegend |
| CD169 | SER-4 | Alexa Fluor 488 | In-house made |
| B220 | 6B2 | Alexa Fluor 405 | In-house made |
| F4/80 | T45-2342 | PE-CF594 | BD Biosciences |
| CD8a | 53-6.7 | PE-Cy7 | BD Biosciences |
| CD11c | HL3 | BV650 | BD Biosciences |
| I-A/I-E | M5/114.15.2 | PE | eBioscience |
| I-A/I-E | M5/114.15.2 | Alexa Fluor 488 | In-house made |
| CD80 | 16-10A1 | PE | Immunotools |
| CD86 | GL-1 | PE-Cy7 | BD Biosciences |
| XCR1 | ZET | BV421 | Biolegend |
| CD40 | 1C10 | Biotin | In-house made |
| CD40L | MR1 | PE-Cy7 | Biolegend |
| CD70 | FR70 | biotin | BD Biosciences |
| CD8a | 53-6.7 | APC | BD Biosciences |
| CD44 | KM81 | FITC | Immunotools |
| H-Kb/SIINFEKL | N/A | PE tetramer | LUMC, Leiden |
| B220 | RA3-6B2 | BV510 | Biolegend |
| CD38 | 90/CD38 | PE | BD Biosciences |
| GL7 | GL-7 | PE-Cy7 | Biolegend |
| OVA | N/A | Alexa Fluor 488 | Invitrogen |
| CD11a | M17/4 | FITC | eBioscience |
| CD8a | 53-6.7 | PE-Cy7 | BD Biosciences |
| CD4 | GK1.5 | PE | eBioscience |
| CD1d PBS-57 | N/A | PE | NIH tetramer core facility |
| IFNγ | XMG1.2 | APC | eBioscience |