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. 2021 Jan 11;11:612230. doi: 10.3389/fphys.2020.612230

FIGURE 2.

FIGURE 2

Pharyngeal arch patterning and ectopic expression of Jag1 in the Hoxb3Tg/+ mutants. (A–F) Wholemount in situ hybridization showing expression of dHAND, Gsc, and Dlx5 in wildtype and Hoxb3Tg/+ embryos at E10.5 (n > 3). (G,H) Wholemount in situ hybridization of Jag1 showing ectopic expression in PA1 and PA2 (arrowheads) of E9.5 Hoxb3Tg/+ embryos (n = 4). (I,J) Coronal sections of embryos subjected to wholemount in situ hybridization of Jag1 showing ectopic expression in the ectodermal epithelium of PA1 and PA2 (arrowheads) in Hoxb3Tg/+ embryos (n = 3). (K,L) Immunostaining for Jag1 (red) and E-cadherin (green) on coronal sections of E9.5 wildtype and Hoxb3Tg/+ embryos (n = 5). (M–P) Wholemount in situ hybridization of Fgf3 and Fgf8 on E9.5 wildtype and Hoxb3Tg/+ embryos (n > 3). PA1, PA2, and PA3 indicate the 1st, 2nd, and 3rd pharyngeal arches. Scale bars: 100 μm.