Figure 12.

Neuroprotective effect of compounds after Ferrous Sulfate (Fe)/L-Ascorbic Acid (AscH(-)) insult. SH-SY5Y cells were treated with Fe/Asc (2.5 M/5 mM, respectively) for 24 h in the absence or in the presence of compounds. When compounds were present, a pre-incubation of 1 h preceded the Fe/Asc treatment. Cellular viability was determined by employing the MTT reduction assay. Results are expressed relatively to SH-SY5Y untreated cells, with the mean ± SEM derived from at least three different experiments. **** p < 0.0001, significantly different when compared with SH-SY5Y untreated cells; # p < 0.05, significantly different when compared with Fe/Asc treated SH-SY5Y cells. (compounds: 1, 2–100 µM; 4–10 µM; 5–20 µM).