Table 1.
List of liposomes used in the study.
| Liposome Description | Lipid Composition | Bulk Buffer Solution | Catalogue nr. | Lipid Stock Concentration (mg/mL) |
|---|---|---|---|---|
| Plain DPPC/CHOL liposomes | DPPC:CHOL (55:55 mol/mol) |
10% sucrose, 20 mM HEPES, <10% ethanol, pH 7.4 | F10102 | 34.6 |
| Plain DSPC/CHOL liposomes | DSPC:CHOL (55:55 mol/mol) |
10% sucrose, 20 mM HEPES, pH 7.4 |
F10103 | 36.5 |
| DSPC/CHOL liposomes with ammonium sulfate gradient |
DSPC:CHOL (55:55 mol/mol) |
10% sucrose, 20 mM NaPO4, pH 6.5 |
F20103A | 42.2 |
| HSPC/CHOL liposomes with ammonium sulfate gradient |
HSPC:CHOL (55:55 mol/mol) |
10% sucrose, 20 mM NaPO4, pH 6.5 |
F20104A | 36.3 |
| DOTAP/DOPE cationic liposomes | DOTAP:DOPE (50:50 mol/mol) |
Nuclease-free water, 10% ethanol, pH 5.5 |
F50102 | 3.6 |
| DC-CHOL/DOPE cationic liposomes | DC-CHOL:DOPE (30:70 mol/mol) |
Nuclease-free water, pH 5.5 |
F50105 | 3.4 |
| Clophosome—high potency clodronate liposomes (neutral) |
PC:CHOL (* mol/mol) |
PBS (0.9% NaCl, 20 mM NaPO4), pH 7.5 |
F70101C-NH | 20.0 |
| Control empty liposomes for Clophosome—high potency (neutral) |
PC:CHOL (* mol/mol) |
PBS (0.9% NaCl, 20 mM NaPO4), pH 7.4 |
F70101-NH | 20.0 |
CHOL, cholesterol; DC-CHOL, 3b-(N-(N′,N′-dimethylaminoethane)-carbamoyl) cholesterol hydrochloride; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOTAP, 1,2-dioleoyl-3-timethylamonium-propane; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DSPC, 1,2-distearoyl-sn-glycero-3-phosphocholine; HSPC, hydrogenated phosphatidylcholine; PC, phosphatidylcholine; PBS, Phosphate Buffer Saline. (* mol/mol), not provided by the supplier and declared as proprietary information. Please note that Clophosome contains 20 mg/mL of clodronate disodium.