FIGURE 5.

DSD inhibited GC cell proliferation and decreased its viability. (A,B) CCK-8 assay was performed to measure cell proliferation rate at 24 and 48 h after DSD treatment at different dosages (0, 50, 100, 200, 300, 400, 600 μg/ml). (C) xCELLigence RTCA was used to evaluate the drug toxicity at 24 h after DSD treatment at 150 μg/ml in SGC-7901 and 165 μg/ml in AGS. (D–G) Cell colony formation assay was performed to measure cell clonality after DSD treatment for 24 h. **p < 0.01 based on the Student t-test. (H–M) The Operetta CLS High Content Analysis System was used to evaluate the accumulated distance of cells at 24 h after DSD treatment at 150 μg/ml in SGC-7901 and 165 μg/ml in AGS.*p < 0.05 based on the Student t-test.