KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 488 | Thermo Fisher Scientific | Cat# 53-0041-82; RRID: AB_469893 |
| CD4 Monoclonal Antibody (GK1.5), APC | Thermo Fisher Scientific | Cat# 17-0041-82; RRID: AB_469320 |
| Rat Anti-CD4 Monoclonal Antibody, Pacific Blue Conjugated, Clone RM4–5 | BD Biosciences | Cat# 558107; RRID: AB_397030 |
| Rabbit Anti-beta-Actin Monoclonal Antibody, HRP Conjugated, Clone 13E5 | Cell Signaling | Cat# 5125; RRID: AB_1903890 |
| NFAT1 (D43B1) XP Rabbit mAb antibody | Cell Signaling | Cat# 5861; RRID: AB_10834808 |
| Anti-NFAT1 (phospho S54) antibody (ab200819) | Abcam | Cat# ab200819 |
| Dynabeads Mouse T-Activator CD3/CD28 | Thermo Fisher Scientific | Cat# 11456D |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LIVE/DEAD Fixable Aqua Dead Cell Stain Kit | Thermo Fisher Scientific | Cat# L34966 |
| CellTrace CFSE Cell Proliferation Kit | Thermo Fisher Scientific | Cat# C34554 |
| Heptelidic Acid | Cayman Chemicals | Cat# 14079 |
| Seahorse XF Cell Mito Stress Test Kit | Agilent | Cat# 103015–100 |
| CD4+CD25+ Regulatory T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat# 130-091-041 |
| Sodium L-lactate | Millipore Sigma | Cat# L7022 |
| Sodium D-lactate | Millipore Sigma | Cat# 71716 |
| Sodium Pyruvate | Thermo Fischer Scientific | Cat# 11360070 |
| Sodium Oxalate | Millipore Sigma | Cat# 223433 |
| L-serine | Millipore Sigma | Cat# S4500 |
| Cell-Tak | BD Biosciences | Cat# 354240 |
| D-GLUCOSE (U-13C6, 99%) | Cambridge Isotope Laboratories | Cat# CLM-1396-PK |
| SODIUM PYRUVATE (13C3, 99%) | Cambridge Isotope Laboratories | Cat# CLM-2440-PK |
| SODIUM L-LACTATE (13C3, 98%) | Cambridge Isotope Laboratories | CLM-1579-PK |
| Calcium Sensor Dye eFluor 514 | Thermo Fisher Scientific | Cat# 65085970 |
| Bovine Serum Albumin Fraction V, heat shock, fatty acid free | Roche | Cat# 3117057001 |
| Riboflavin 5-monophosphate sodium salt hydrate | Millipore Sigma | Cat# F6750 |
| Resazurin sodium salt | Millipore Sigma | Cat# R7017 |
| Diaphorase from Clostridium kluyveri | Millipore Sigma | Cat# D5540 |
| Alcohol Dehydrogenase from Saccharomyces cerevisiae | Millipore Sigma | Cat# A3263 |
| NAD | Roche | Cat# 10127965001 |
| NADH | Roche | Cat# 10004634103 |
| Critical Commercial Assays | ||
| L-Lactate Assay Kit | Cayman Chemicals | Cat# 700510 |
| iSTAT® CG4+ cartridge | Abbott | Cat# 03P85–25 |
| Experimental Models: Cell Lines | ||
| C2C12 mouse myoblast cell line | American Type Culture Collection | ATCC Cat# CRL-1772, RRID:CVCL_0188 |
| Ae17 mouse mesothelioma, sOVA | (Jackaman et al., 2003) | RRID:CVCL_LJ85 |
| Experimental Models: Organisms/Strains | ||
| B6.129S7-Rag1tm1Mom/J (B6/Rag1−/−) | The Jackson Laboratory | Cat# JAX:002216, RRID:IMSR_JAX:002216 |
| C57BL/6J | The Jackson Laboratory | Cat# JAX:000664, RRID:IMSR_JAX:000664 |
| BALB/cJ | The Jackson Laboratory | Cat# JAX:000651, RRID:IMSR_JAX:000651 |
| Software and Algorithms | ||
| ImageJ64 | NIH | https://imagej.nih.gov/ij/download |
| Wave 2.6.1 | Agilent | https://www.agilent.com |
| Image Lab 5.2.1 | Bio-Rad | https://www.bio-rad.com |
| Other | ||
| RPMI 1640 Medium | Thermo Fisher Scientific | Cat# 11875085 |
| Fetal Bovine Serum, certified, United States | Thermo Fisher Scientific | Cat# 16000044 |
| RPMI 1640 medium, no glucose | Thermo Fisher Scientific | Cat# 11879020 |
| Fetal Bovine Serum, dialyzed, US origin | Thermo Fisher Scientific | Cat# 26400044 |
| Teknova RPMI-1640 Media without Glucose, Glycine and Serine | Teknova | Cat# 50-190-8105 |
| DMEM, high glucose, GlutaMAX(™), pyruvate | Thermo Fisher Scientific | Cat# 10569 |
| Seahorse XF Media & Calibrant | Agilent | Cat# 102353–100 |
| CELLview culture dish | Greimer Bio-One | Cat# 627870 |
| Microhematocrit Capillary Tubes, Heparinized | Thermo Fisher Scientific | Cat# 22-362-566 |