Skip to main content
. Author manuscript; available in PMC: 2021 Jan 25.
Published in final edited form as: Mol Pharm. 2017 Jun 20;14(7):2368–2377. doi: 10.1021/acs.molpharmaceut.7b00277

Figure 3.

Figure 3.

Effect of PF-4989216 on the transport function of ABCG2. The accumulation of fluorescent pheophorbide A (PhA) in drug-sensitive S1 (A, left panel) and ABCG2-overexpressing S1-M1–80 (A, right panel); drug-sensitive MCF7 (B, left panel) and ABCG2-overexpressing MCF7-FLV1000 (B, right panel); drug-sensitive human nonsmall lung carcinoma H460 (C, left panel) and ABCG2-overexpressing H460-MX20 MDR (C, right panel) cell lines; or parental HEK293 (D, left panel) and ABCG2-transfected R482-HEK293 cells (D, right panel), were measured in the absence (solid lines) or presence of 40 μM PF-4989216 (shaded, solid lines) or 1 μM ABCG2 reference inhibitor Ko143 (dotted lines), and analyzed immediately by flow cytometry. Representative histograms of three independent experiments are shown.