Figure 5.

Cell migration, collagen synthesis ability, and store-operated Ca²⁺ entry in human cardiac fibroblasts treated with LiCl (0.1 mM) and 2-APB (10 μM) for 24 h. (A) Photographs and average data of the migration assay in human cardiac fibroblasts (n = 7 from four independent experiments). (B) Photographs and average data of the pro-collagen type IA1 production (n = 5 from three independent experiments) in human cardiac fibroblasts. (C) Representative tracings and average data of the maximum amplitude and AUC of intracellular Ca²⁺ concentration following the re-addition of extracellular Ca²⁺ (2 mM) in control, LiCl (0.1 mM), 2-APB (10 μM), or combined lithium (0.1 mM) and 2-APB (10 μM)-treated human cardiac fibroblasts (n = 3 independent experiments). One-way repeated measures analysis of variance (ANOVA) test with Tukey’s post hoc test was used to compare the human cardiac fibroblasts under different treatment conditions. * p < 0.05, ** p < 0.01, *** p < 0.001.