Table 1.

Overview of the protocols developed for the decellularization of organ fragments and whole organs.

Organ Fragments	Protocol	Reference
Porcine testis	−80 °C, 0.01% SDS, 1% Triton X-100	[159]
Goat lung	0.25%Trypsin-EDTA, 0.1% SDS, 20 μg/mL RNase A and 0.2 mg/mL DNase I	[160]
Human lung	8 mM CHAPS 1	[154]
Porcine myocardium	0.1% SDS, 0.01% Trypsin, 1 mM PMSF 2, 20 μg/mL RNase A and 0.2 mg/mL DNase I	[161]
Porcine liver	0.01% SDS, 1% Triton X-100	[162]
Porcine kidney	1% Triton X-100	[163]
Whole Organ	Protocol	Reference
Bovine ovaries	0,1% SDS	[164]
Porcine ovaries	−80 °C, 0.5% SDS, 1% Triton X-100, 2% SD	[155]
Ovine testis	1% SDS	[165]
Human trachea	4% SD, 2000 kU DNase I in 1 mM NaCl	[166]
Porcine trachea	4% SD, 2 kU/mL DNase I in 1 M NaCl	[167]
Porcine lung	−80 °C, 2% SDS, 1% SDS	[168]
Human lung	1% Triton X-100, 0.5% SDS	[153]
Porcine heart	0.2% Trypsin/0.05% EDTA/0.05% NaN3, 3% Triton X-100/0.05% EDTA/0.05% NaN3, 4% SD	[169]
Rabbit liver	ddH2O, 1% Triton X/0.1% NH4OH	[170]
Porcine liver	1% SDS, 1% Triton X-100, 1% SD	[171]
Porcine cornea	0.5% SDS	[147]
Goat kidney	50 U/mL heparin, 0.1% SDS, 0.5% SDS, 1% SDS, 0.1% Triton X-100, 5 mM CaCl2 and MgSO4, 0.2 mg/mL DNase I	[172]

¹ CHAPS = 3-((3-Cholamidopropyl)-dimethylammonio)-1-propanesulfonate. ² PMSF = phenylmethylsulfonylfluoride.