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(a) Molecular structure of 8-anilino-1-naphthalenesulfonic acid (ANS). (b) Normalized absorption and fluorescence spectra of ANS in water, as well as normalized fluorescence spectra solutions of lysozyme (1.0 mg/mL) and bovine serum albumin (BSA) (1.0 mg/mL). (c) Comparison of the fluorescence spectrum of BSA (1.0 mg/mL) solution with that of water and lysozyme solution.
