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. 2021 Jan 15;10(1):156. doi: 10.3390/cells10010156

Figure 1.

Figure 1

Rocuronium activates mouse peritoneal mast cells (PMCs) via MrgprB2. (A) PCR analysis was performed to determine the mRNA expression of MrgprB2 in wild-type (WT) and MrgprB2−/− PMCs. GAPDH was used for normalization. (B) WT and MrgprB2−/− PMCs were stained with Toluidine blue and Alcian/Safranin. The images were acquired at 40× resolution. Bars = 10 and 20 μm. (C) WT and MrgprB2−/− PMCs were stimulated with rocuronium (20 μg/mL) for 30 min, and β-hexosaminidase release was determined. Data are expressed as mean ± SEM. Statistical significance was determined by two-way ANOVA with Tukey post-hoc test. **** p < 0.0001 compared to the control, #### p < 0.0001 compared between WT and MrgprB2−/− groups.