Figure 3.

Biolayer interferometry analysis of the interaction between APR peptides and S100B. (a) The synthetic APR-S100B peptides were C-terminal acetylated and linked to a PEG-biotin tag which served to link the peptides to a streptavidin BLI biosensor; (b) BLI binding interferograms representative of at least 3 independent traces. Experiments were carried out at a fixed S100B concentration (50 μM) with 50 mM HEPES pH 7.4, 150 mM NaCl, 0.01% Tween 20 at 25 °C.