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. 2021 Jan 15;10(1):169. doi: 10.3390/cells10010169

Figure 1.

Figure 1

CD112 is expressed by endothelial cells in vivo. (A,B) Flow cytometry analysis of mouse ear skin single-cell suspensions. (A) Endothelial cells were identified as CD31+CD45− cells (left) and further divided into blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) based on podoplanin (podo) expression (right). (B) Representative flow cytometry plot showing in vivo expression of CD112 by LECs (green; CD31+podo+) and BECs (red; CD31+podo−). (C) Summary of median fluorescent intensity (MFI) values of CD112 expression on murine LECs and BECs of three experiments. Data points from the same experiment are connected by a line. (D) Low magnification confocal images of ear skin whole-mount immunofluorescence staining visualizing CD112 expression (white) by lymphatic vessels (LVs) and blood vessels (BVs), indicated by white arrow heads. Scale bar: 50 μm. (E) High magnification confocal images revealed a button-like expression pattern for CD112 (white) in lymphatic capillaries (LYVE-1+/VE-cadherin+ upper panel), respectively, and a zipper-like expression pattern in lymphatic collectors (LYVE-1−/VE-cadherin+ lower panel). Scale bar: 20 μm.