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. 2021 Jan 17;22(2):884. doi: 10.3390/ijms22020884

Figure 1.

Figure 1

Representative sensorgrams of the BHD1028-AdipoRs binding overlay plot with the kinetic fit. The BHD1028-receptor binding sensorgrams were obtained after injection of various concentrations of BHD1028 into an AdipoR1-Δ88 and AdipoR2-Δ99 on the immobilized CM5 chip at pH 5.0. Concentrations titrated at a 1:1 ratio with red being the highest concentration and blue the lowest concentration flowing at 30 μL/min with a total association time of 180 s and disassociation of 300 s. The data were presented in types of the kinetic model: The equilibrium dissociation constant at the heterogeneous ligand model ((A) AdipoR1-BHD1028. (B) AdipoR2-BHD1028). SPR signals from different concentrations were plotted against concentrations to calculate a Kd value.