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SFN inhibited the accumulation of autophagic light chain 3 (LC3) and Beclin1 within osteoclasts. RAW264.7 cells were induced by 50 ng/mL of RANKL for 5 days, and immunofluorescence (IF) analysis was performed. (a) Representative IF images locating LC3 (green) within the osteoclasts. (b) Representative IF images locating Beclin1 (green) within the osteoclasts. F-actin was stained with phalloidin (red). DAPI (blue) was used to visualize the nuclei.
