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The administration of SFN attenuated autophagic LC3 expression in an LPS-induced mouse calvaria model. Calvarias were fixed, decalcified, dehydrated, embedded, and sectioned. (a) Representative IHC images locating LC3. (b) Representative IF images of the distribution of LC3 (green). Phalloidin (red) indicates cytoskeleton and bone. DAPI (blue) was used to counterstain the nuclei.
