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. 2021 Jan 14;10(1):112. doi: 10.3390/antiox10010112

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell death, ROS production, and lipid peroxidation in zebrafish. Zebrafish embryos were pre-treated with TLE (50, 100, and 200 μg/mL) and/or treated with H₂O₂ (5 mM). Imaging of (a) cell death, (b) ROS production, and (c) lipid peroxidation was performed using fluorescence microscopy. The fluorescence intensity of zebrafish was quantified using ImageJ software. The data were measured in triplicate and expressed as the mean ± SE. * p < 0.05 compared with the H₂O₂-treated group.

Cell death, ROS production, and lipid peroxidation in zebrafish. Zebrafish embryos were pre-treated with TLE (50, 100, and 200 μg/mL) and/or treated with H₂O₂ (5 mM). Imaging of (a) cell death, (b) ROS production, and (c) lipid peroxidation was performed using fluorescence microscopy. The fluorescence intensity of zebrafish was quantified using ImageJ software. The data were measured in triplicate and expressed as the mean ± SE. * p < 0.05 compared with the H₂O₂-treated group.