Figure 5.

The direct bind between four miRNA members from miR-959–962 cluster and its target genes were confirmed by Dual luciferase reporter assay in vitro. (A,D,G) The schematic diagrams of construction of targets 3′ UTR and 3′ UTR mutation luciferase reporter plasmids were presented. After co-transfected with miRNA expression plasmid, the corresponding luciferase activity of the report plasmids without or with mutation sites was determined in Drosophila S2 cell on a Dual luciferase assay (B,C,E,F,H). (** p < 0.01; *** p < 0.001; and ns, no significance vs. the control).