Figure 4.
Glucose, lactate, pyruvate, aspartate, glutamine, glutamate, alanine and serine conversion rates in uninfected and C. parvum-infected HCT-8 cultivated in glutamine-starved medium (0.02 mM) at hyperoxia (21% O2) and physioxia (5% O2) (n = 6, each group). In comparison to glutamine-supplemented cultivation conditions the parasite infection rate slightly dropped in glutamine starved medium (21% O2: 40 ± 3%; 5% O2: 28 ± 7%). Conversion rates were analysed at 48 h p. i. in the cell culture supernatants of the cells. Metabolic conversion rates are plotted as interleaved box and whiskers plots showing minimum to maximum values with line at the medians. Statistical significance (* = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001, **** = p ≤ 0.0001) was determined by t-test for independent samples.