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Effect of C. parvum infection on glycolysis and glycolytic ATP production in HCT-8 cells under hyperoxic (21% O2) conditions. (a) C. parvum infection-triggered upregulation of glycolysis in HCT-8 cells as evidenced by Seahorse Glycolysis Stress Test Kit. (b) Likewise, significant infection-induced upregulation of glycolysis-derived ATP was observed. Bar graph shows mean ± SD, (n = 3). t-test was use for evaluation of significance (* = p ≤ 0.05, ** = p ≤ 0.01).
