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. 2021 Jan 18;26(2):479. doi: 10.3390/molecules26020479

Table 2.

The retention times of the six most important chromatographic peaks in HPLC and their corresponding MS2 mass spectra inference information.

Peak a RT (min) MS2 Mass Spectrum [M − H] Molecular Formula Proposed Compound
1 10.29 Figure 2B 301.2167 C20H30O2 EPA
2 11.26 Figure 2C 277.2168 C18H30O2 ALA
3 12.19 Figure 2D 327.2279 C22H32O2 DHA
4 13.91 Figure 2E 303.2325 C20H32O2 ARA
5 15.66 Figure 2F 279.2287 C18H32O2 LA
6 22.53 Figure 2G 281.2446 C18H34O2 OA

a The number of chromatographic peak in HPLC chromatogram (Figure 1C); RT: the retention time of chromatographic peaks in HPLC; EPA: eicosapentaenoic acid; ALA: α-linolenic acid; DHA: docosahexaenoic acid; ARA: arachidonic acid; LA: linoleic acid; OA: oleic acid.