Table 4.
The robustness test results of relative correction factors (RCF) for five unsaturated fatty acids (UFAs) by Taguchi design–static design.
| HPLC Instruments | Chromatographic Columns | FLA/LA | FLA/EPA | FLA/ALA | FLA/DHA | FLA/ARA | FLA/OA |
|---|---|---|---|---|---|---|---|
| Agilent-UVD | Agilent | 1 | 2.5228 | 1.4316 | 3.2654 | 2.4059 | 0.1999 |
| Agilent-UVD | Waters | 1 | 2.5132 | 1.4023 | 3.2009 | 2.4014 | 0.2031 |
| Agilent-UVD | Venusil | 1 | 2.5058 | 1.4180 | 3.1377 | 2.3661 | 0.2003 |
| Agilent-DAD | Agilent | 1 | 2.5104 | 1.4052 | 3.1953 | 2.3889 | 0.1994 |
| Agilent-DAD | Waters | 1 | 2.5033 | 1.4023 | 3.2901 | 2.4105 | 0.1973 |
| Agilent-DAD | Venusil | 1 | 2.5123 | 1.4193 | 3.2195 | 2.4190 | 0.1983 |
| Waters-E2695 | Agilent | 1 | 2.5916 | 1.4329 | 3.2130 | 2.4395 | 0.1939 |
| Waters-E2695 | Waters | 1 | 2.5412 | 1.4032 | 3.2014 | 2.4511 | 0.2031 |
| Waters-E2695 | Venusil | 1 | 2.4994 | 1.3857 | 3.1959 | 2.3498 | 0.1942 |
| Mean | 1 | 2.5222 | 1.4112 | 3.2132 | 2.4036 | 0.1988 | |
| RSD (%) | 0 | 1.08 | 1.03 | 1.28 | 1.27 | 1.58 | |
EPA: eicosapentaenoic acid; ALA: α-linolenic acid; DHA: docosahexaenoic acid; ARA: arachidonic acid; LA: linoleic acid; OA: oleic acid; RSD: relative standard deviation; FLA/EPA, FLA/ALA, FLA/DHA, FLA/ARA, and FLA/OA are the corresponding RCF of EPA, ALA, DHA, ARA, and OA, respectively; RSD: relative standard deviation.