Table 5.
Cabeza gain of function and RNAi-induced cabeza silencing phenotypes.
| Gal4 Line | Line | Phenotype/Reference |
|---|---|---|
| Act5C-Gal4 (ubiquitous) |
UAS-caz | Lethal, no adult eclosion [160] |
| UAS-RNAi caz (363–399) VDRC 100291 |
Low adult eclosion [160] No effect at 28 °C [150] |
|
| UAS-RNAi caz (1–167) |
Lethal at 28 °C, no effect at 25 °C [150] Late pupal lethality [176] |
|
| UAS-RNAi caz (180–346) |
No effect at 28 °C [150] | |
| Elav-Gal4 (pan neuronal) |
UAS-caz | Almost lethal at pupal stage, few escapers [160] |
| UAS-RNAi caz (363–399) VDRC 100291 |
Adult eclosion defect [160] No effect on lifespan, reduced mobility from young adult, decreased in total axonal branch length [150] and synaptic bouton number [177] Adult climbing defect, number of synaptic bouton and total branch length reduced [178,179] |
|
| UAS-RNAi caz (1–167) |
No effect on lifespan, reduced mobility from young adult. In larvae, decreased synaptic bouton number [150,177] and decreased total axonal branch length [150] | |
| UAS-RNAi caz (180–346) |
No effect on lifespan [150] | |
| D42-Gal4 (motoneurons) |
UAS-Caz | Pupal lethality, no adult eclosion [160] |
| UAS-RNAi caz (363–399) VDRC 100291 |
Low adult eclosion [160] | |
| OK6-Gal4 (motoneurons) |
UAS-caz | Increased synaptic bouton number [159] Rescued the caz1 phenotypes [159] |
| OK371-Gal4 (glutamatergic neurons) |
UAS-caz | Severe larval crawling defect, reduction of synaptic bouton number [160] |
| GMR-Gal4 (eye) |
UAS-caz | Rough eye [160,177] |
| UAS-RNAi caz (1–167) |
Rough eye [176,178] | |
| UAS-RNAi caz (363–399) VDRC 100291 |
Severe rough eye [179,180] apoptosis [176] | |
| nsyb-Gal4 (pan neuronal) | UAS-RNAi caz (363–399) VDRC 100291 |
No significant lethality, slight motor defect [175] |
| UAS-RNAi HMS00790 | No significant lethality, slight motor defect [175] | |
| UAS-RNAi HMS00156 | No significant lethality, slight motor defect [175] | |
| Ppk-Gal4 (da neuron) |
UAS-caz | Reduced synaptic projections [181] |
| UAS-caz P398L | Reduced synaptic projections [181] |
This table describes the phenotypes associated with different UAS-cabeza or UAS RNAi cabeza lines. Note that the experiments could be done at different temperatures and the UAS lines were generated using different genetic strategies (site-specific or random insertion).