Table 8.
Gal4 Line | RNAi Line | Phenotype/Reference |
---|---|---|
Act5C-Gal4 (ubiquitous) |
104401 (VDRC) | Lethal [269] |
38377 (VDRC) | Lethal [269] Adult flies with ectopic bristles [287] |
|
Tubulin-Gal4 (ubiquitous) |
38377 (VDRC) | Lethal pupal with adult escapers [274] |
38379 (VDRC) | Lethal pupal with adult escapers [274] | |
Homemade RNAi targeting exon 5 | Larval/pupal lethality with adult escapers, impaired adult motor behavior [266] | |
Tubulin-Gal4, Tubulin-Gal80TS (ubiquitous, induced at adult stage) |
38379 (VDRC) | Climbing defect, reduced lifespan [288] |
Elav-Gal4 (pan-neuronal) |
38377 (VDRC) | Impaired larval locomotion [265] Age progressive climbing deficit [267,269] Reduced lifespan [269] |
38379 (VDRC) | Age progressive climbing deficit [267] | |
104401 (VDRC) | Age progressive climbing deficit, reduced lifespan [269] | |
29517 (BDSC) | Age progressive climbing deficit [269] | |
39014 (BDSC) | Age progressive climbing deficit [269] | |
Homemade RNAi targeting exon 5 | Impaired synaptic transmission (increase quantal content) at larval NMJ and abnormal adult motor behavior [266] | |
Elav-Gal4, Tubulin-Gal80TS (pan-neuronal, induced at larval stage) |
38379 (VDRC) | Abnormal distribution of Dlg and GluRIIA at larval NMJs [288] |
1407-Gal4 (pan-neuronal) | 38377 (VDRC) | Strong climbing defect [267] |
D42-Gal4 (motoneurons) |
38377 (VDRC) | Normal larval locomotion [265] Increased larval turning, age progressive climbing deficit [289] |
38379 (VDRC) | Increased larval turning, age progressive climbing deficit [289] | |
OK371-Gal4 (glutamatergic neurons) |
38377 (VDRC) | Normal larval locomotion [290] |
OK371-Gal4/MARCM (individual leg motoneuron) |
38379 (VDRC) | Normal larval locomotion [290] |
Unknown | No effect on leg motoneuron/axon [291] | |
Repo-Gal4 (pan-glial) |
38379 (VDRC) | Larval locomotion and climbing defects, reduced lifespan, wrapping defect at larval NMJ [271] |
Homemade RNAi targeting exon 5 | Normal lifespan and climbing, impaired overall motor activity in 30 day old flies [270] | |
BG57-Gal4 (muscles) |
Homemade RNAi targeting exon 5 | Impaired synaptic transmission at larval NMJ (normal quantal content) [266] |
Mef2-Gal4 (muscles) |
38379 (VDRC) | Impaired larval locomotion and climbing, reduced lifespan [282] |
Homemade RNAi targeting exon 5 | Normal lifespan and climbing, impaired overall motor activity in 30 day old flies [270] | |
Mhc-Gal4 (muscles) |
38377 (VDRC) | Normal larval locomotion [265] Impaired larval locomotion and climbing [282] |
GMR-Gal4 (eye) |
38377 (VDRC) | No effect [290] Mild rough eye [269,292] |
38379 (VDRC) | No effect [290] | |
104401 (VDRC) | Mild rough eye [269] | |
29517 (BDSC) | Mild rough eye [269] | |
39014 (BDSC) | Mild rough eye [269] | |
Unknown | No effect [272], weak eye degeneration [293] | |
EB1-Gal4 (ellipsoid body neurons) |
Homemade RNAi targeting exon 5 | Impaired adult motor behavior, age dependent axonal/synaptic degeneration and loss of upper motoneuron [266] |
Gal4221-Gal4 (sensory neurons) |
38377 (VDRC) | Reduced dendritic branching [274] |
38379 (VDRC) | Reduced dendritic branching [274] | |
OK107-Gal4 (mushroom bodies) |
38377 (VDRC) | Normal mushroom bodies, weak learning inability [265] Axonal loss and neuronal death [290] |
38379 (VDRC) | Normal mushroom bodies and learning ability [265] Axonal loss and neuronal death [290] |
This table describes the Gal4 lines used to express TBPH RNAi, the type/origin of RNAi construct, and the associated phenotypes/references. It is important to note that all experiments were not done at the same temperature. In addition, some experiments have combined RNAi and Dicer-2 expression to enhance the effect of the RNAi (not mentioned). Altogether, these different experimental conditions may explain some discrepancies. Data obtained at larval NMJs are not described.