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. 2021 Jan 18;19(1):41. doi: 10.3390/md19010041

Figure 1.

Figure 1

The overall design of the stepwise drug screen. (A) The path towards identifying topsentinol L trisulfate (TLT). Marine sponge PNG07-3-073 was diced and soaked in methanol to obtain a crude extract, which was fractionated on HP20SS resin. Among the five fractions obtained, only the F2 fraction exhibited tumoricidal activity (Screen 1); (B) The viability data for fraction PNG07-3-073-F2, this activity was observed against basal-like and claudin-low (BL-CL) cell lines (Screen 2). Then, PNG07-3-073-F2 was further fractionated, and the subfractions investigated for anti-BL-CL activity, where the M6 fraction was identified as being BL-CL selective (Screen 3); (C) The viability data for fraction PNG07-3-073-F2-M6. Large-scale isolation of PNG07-3-073 ensued, culminating in the purification of TLT and its identification as the active compound in PNG07-3-073 responsible for anti-BL-CL effects (Table 1). Cell lines were treated and the bar graphs (with standard deviation) with all data displayed as scatter dot diagrams of the viability assessment of each cell line.