. 2021 Jan 25;25:36. doi: 10.1186/s13054-020-03424-1

Table 4.

List of primers for quantification of mRNA expression of mitochondrial quality mechanisms, mitochondrial complexes and oxidation pathways by RT-qPCR

Gene	Primer sequence		Process
PINK1	Forward:	GACGCTGTTCCTCGTTATGA	Mitophagy
PINK1	Reverse:	TCCAGCTCCACAAGGATGTT	Mitophagy
PARKIN	Forward:	GACCCTCAACTTGGCTACTC	Mitophagy
PARKIN	Reverse:	CTTCGCAGGTGACTTTCCTC	Mitophagy
MFN2	Forward:	CCGCCACATAGAGGAAGGAC	Fusion
MFN2	Reverse:	CGCACAGACACAGGAAGGAG	Fusion
DRP1	Forward:	AAGCTGCTGCCATAGTCCTC	Fission
DRP1	Reverse:	ACCACAGCCATGTCAGTGTC	Fission
NRF2	Forward:	GCTACTAATCAGGCTCAGTC	Mitochondrial biogenesis
NRF2	Reverse:	GTAGTCTCAACCAGCTTGTC	Mitochondrial biogenesis
TFAM	Forward:	CATGGACTTCTGCCAGCATA	Mitochondrial biogenesis
TFAM	Reverse:	AGAACACCGTGGCTTCTACA	Mitochondrial biogenesis
mnSOD	Forward:	ACGCGGCCTACGTGAACAAC	Antioxidant enzyme
mnSOD	Reverse:	CAACAGATGCAGCCGTCAGC	Antioxidant enzyme
OGG1	Forward:	GTGTGCGACTGCTGCGACAA	mtDNA damage repair (excision of 8-oxoguanine)
OGG1	Reverse:	CTGGATGAGCCGAGGTCCAA	mtDNA damage repair (excision of 8-oxoguanine)
HIF1α	Forward:	TGAGGGGACAGGAGGATCAG	Master regulator of cellular and systemic homeostatic response to hypoxia
HIF1α	Reverse:	CACGCGGAGAAGAGAAGGAA
SIRT1	Forward:	ATGCTGGCCTAATAGAGTGG	Regulates epigenetic gene silencing, biogenese and antioxidant mechanisms
SIRT1	Reverse:	TCTGGAACATCAGGCTCATC
NGAL	Forward:	GGTGAGCACCAACTACAACC	Early biomarker of acute kidney injury
NGAL	Reverse:	GCCCAGAGATTTGGAGAAGC	Early biomarker of acute kidney injury
NDUFA1	Forward;	ACTGGCTACTGCGTACATCC	ETC complex 1 (nDNA)
NDUFA1	Reverse:	AGATGCGCCTATCTCTTTCC	ETC complex 1 (nDNA)
COX5b	Forward:	CATTGGCTCCTTCTCCCATA	ETC complex 4 (nDNA)
COX5b	Reverse:	CATACCAGGTGGTCCCATTC	ETC complex 4 (nDNA)
B-actin	Forward:	AGGATGCAGAAGGAGATCAC	Housekeeping gene
B-actin	Reverse:	AGTCATAGTCCGCCTAGAAG	Housekeeping gene

mtDNA mitochondrial DNA, ETC electron transport chain, nDNA nuclear DNA