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. 2021 Jan 25;12:80. doi: 10.1186/s13287-020-02125-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of SKPs and SKP-SCs. a SKPs in floating culture showed spherical colonies. b SKP-derived SCs showed spindle-like shape with side-by-side alignment. c Immunofluorescence staining of SKP spheres showed positive expression of intermediate filament Nestin (green) and chondroitin sulfate proteoglycan Versican (red) with proliferation marker Ki67 (red) and Hoechst (blue) labeled cell nuclei. d SKP-derived SCs showed positive expression of SC markers S100 (red), GFAP (green), and p75^NTR (green), and cell nuclei were labeled with Hoechst (blue). Scale bar, 50 μm