Table 3.

mNGS and r(RT-)PCR results of 7 patients with clinically unrecognized viral sequences

	Viral species detected with mNGS	Viral species confirmed with r(RT)-PCR assays
Patient’s code	Virus	Plasma, n (pos/total)	Time period (days)	Estimated viral load (mean log10 copies/ml plasma or CT values)	Other positive biological specimen
Ge02	HPyV7	14/14	279	4.75	BM, BAL
Ge05	Usutu virus	2/5	7	4.66	–
Ge07	Cutavirus	6/10	98	37.9	Skin
Ge09	Bufavirus	6/12	81	3.73	Stools, duodenum
Ge12	Novel human astrovirus MLB2	2/6	14	3.24	Intestine, colon, BM
Ge14	Rubella virus	3/5	39	37.9	–
	Classic human astrovirus	5/11	25	5.56	–
	HPyV6	11/11	74	3.76	–
Ge22	Rubella virus	2/8	3	36.7	–

Pos/total corresponds to the number of positive samples on the total of screened samples. Time period corresponds to the time period during which samplings were found positive. CT values above 40 were considered negative. CT values are indicated in italics

Abbreviations: BM bone marrow, BAL bronchoalveolar lavage, CT cycle threshold