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. 2021 Jan 25;40:43. doi: 10.1186/s13046-021-01839-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — cMYC mediates the miR-155-driven PTGES2 transactivation. a, b Promoter activity of PTGES2 gene in MDA-MB-436 (a) and Hs-578 T cells (b) with (miRZIP155) or without (control) miR-155 knockdown. PGL3 empty vector was used as negative control. c Schematic diagram showing the cMYC-binding site (E-box, marked in grey) on PTGES1 or PTGES2 gene promoter. Numbers indicate nucleotide positions counted from the transcription start site. d Chromatin immunoprecipitation result of cMYC in MDA-MB-436 control and knockdown (miRZIP155) cells. Promoters of PTGES1 and PTGES2 are tested. IgG was used as negative control. e, f Promoter activity of PTGES2 gene in MDA-MB-436(e) and Hs-578 T cells (f) with miR-155 knockdown (miRZIP), in combination with cMYC reconstitution (miRZIP155 + cMYC). Data on the right panels confirms cMYC protein overexpressed in the reporter assay.g Western blot results of cMYC in the miR-155-high and -low TNBC specimens (n = 20)