Figure 1.

CK2 expression and activity in SK-N-BE and U2OS cells. (A) Titration of the antibody reactivity towards CK2 catalytic subunits. The indicated amounts of recombinant CK2 catalytic subunits (myc-α’ or α) were loaded on SDS-PAGE, and either blotted for the WB (western blot) analysis or stained by Colloidal Coomassie Blue; (B) CK2 expression and activity in w.t. and KO clones of the cells used for this study. 10 μg proteins from cell lysates were analyzed by WB with the indicated antibodies. The last two right lanes belong to an independent experiment. As a reporter of CK2 endogenous activity, pS129 Akt signal has been quantified, normalized to total Akt signal, and reported in the bar graph as % of w.t. cells. At least three independent experiments were performed; representative western blots are shown, while quantification in the bar graphs reports the mean values ± SEM of all experiments and of the two clones of the same KO. Statistical significance refers to w.t. cells. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001