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Proliferation curves of w.t. and KO clones of SK-N-BE and U2OS cells. The cell number was evaluated every 24 h, by means of Trypan blue exclusion assay (see methods for details). Experiments were performed in triplicates; at least three independent experiments were performed. The curve for each KO reports the means values ± SEM obtained with the different clones of the same KO. Significance calculated at 96 h refers to w.t. cells. (***) p < 0.001
