Figure 7.

Lactate production in response to CK2 knock out or pharmacological inhibition. (A) The extracellular lactate secreted in the culture medium in 8 h by the indicated cells was analyzed by means of a luminescence assay. Values for each KO are the means ± SEM obtained with the different clones of the same KO. At least three independent experiments were performed, in duplicate. Significance refers to w.t. cells (B) Cells were analyzed for the extracellular lactate secreted in the culture medium in 6 h, in the absence or in the presence of the indicated concentrations of CX-4945. Three independent experiments were performed, in duplicate. Results (means ± SEM) are reported as % compared to the respective w.t. cells, in the absence of CX-4945. Significance refers to untreated control. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001.