Table 1.
Evaluation for effect of treatment across all measures, with results reported from the a priori statistical approach (Mann–Whitney U) alongside post-hoc parametric testing (2-way ANOVA). Normality was assessed using the Shapiro-Wilk test. Comparable results were found across all measures except for total distance during open field activity assessment, for which Mann–Whitney U found a significant effect of treatment but ANOVA did not. The data for this measure were not normally distributed, so assumptions of ANOVA are not met.
| Mann–Whitney U | Normal distribution | Two-way ANOVA | |||
| Study measure | U-statistic | P-value | Yes/No | F (DFn, DFd) | P-value |
| Elevated zero maze | |||||
| Percent time in open quadrants | 248.5 | 0.42 | Yes | F (1, 44) = 1.068 | 0.31 |
| Total distance traveled (cm) | No | F (1, 44) = 2.655 | 0.11 | ||
| Males | 41 | 0.08 | |||
| Females | 51 | 0.24 | |||
| Open field activity | |||||
| Percent time in central zone | Yes | F (1, 44) = 0.1518 | 0.70 | ||
| Males | 66 | 0.76 | |||
| Females | 57 | 0.41 | |||
| Total distance traveled (cm) | 157 | 0.01 | No | F (1, 44) = 1.284 | 0.26 |
| Fecal boli (no. pellets) | 170.5 | 0.01 | Yes | F (1, 43) = 5.183 | 0.03 |
| Neurogenesis | |||||
| Percent of total cells BrdU-positive | 14.5 | 0.13 | Yes | F (1, 11) = 2.164 | 0.17 |
| BrdU-positive cells by area (no. cells) | 18 | 0.27 | Yes | F (1, 11) = 2.183 | 0.17 |
| Body weight gain (g/course of study) | No | F (1, 43) = 8.929 | 0.005 | ||
| Males | 19 | 0.0014 | |||
| Females | 64 | 0.93 | |||
| Adrenal gland weight (mg) | No | F (1, 43) = 0.01101 | 0.76 | ||
| Males | 67 | 0.78 | |||
| Females | 61.5 | 0.79 | |||
| Plasma corticosterone concentration (ng/mL) | No | F (1, 43) = 0.002178 | 0.96 | ||
| Males | 68 | 0.84 | |||
| Females | 51 | 0.38 | |||
| Neutrophil:lymphocyte ratio | 242.5 | 0.48 | Yes | F (1, 43) = 0.5352 | 0.47 |