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. Author manuscript; available in PMC: 2021 Jun 19.
Published in final edited form as: ACS Chem Biol. 2020 May 6;15(6):1650–1661. doi: 10.1021/acschembio.0c00297

Figure 5.

Figure 5.

Cytosolic uptake of kB1 residue mutants and grafted peptides. Uptake was measured for chloroalkane tagged peptides using CAPA. (A) Results for kB1 analogues with mutations in loop 6. (B) Results for kB1 analogues with grafted epitopes in loop 6. [ct]Tat-R, [ct]Tat-G, and [16ct]kB1 were included as controls. CP50 values of peptides are shown in the right panel. The data were normalized using a no-pulse control as 100% signal and a no-pulse/no-chase control as 0% signal. All experiments were done in triplicate, and the results are represented as mean ± SEM.