Table 2.
Names of Kalata B1 (kB1) Analogues and Their Loop 6 Amino Acid Sequences and Compositions
| Namea | Loop 6 Sequenceb | Hydrophobicc | Negatived | Positivee | Polarf |
|---|---|---|---|---|---|
| Native | |||||
| kB1 | TRNGLPV | 3 | 0 | 1 | 3 |
| Residue Mutants | |||||
| [Rmut, 16]kB1 | TRRGRPR | 1 | 0 | 4 | 2 |
| [Hmut, 16]kB1 | TRHGHPH | 1 | 0 | 4 | 2 |
| [Wmut, 16]kB1 | TRWGLPV | 4 | 0 | 1 | 2 |
| [Emut, 16]kB1 | TREGEPE | 1 | 3 | 1 | 2 |
| [Smut, 16]kB1 | TRSGSPS | 1 | 0 | 1 | 5 |
| [Gmut, 16]kB1 | TRGGGPG | 1 | 0 | 1 | 5 |
| Grafted Peptides | |||||
| [graft1, 16]kB1 | TRGFEWLDWEFGLPV | 8 | 3 | 1 | 3 |
| [graft2, 16]kB1 | TRGLDEETGEFGLPV | 5 | 4 | 1 | 5 |
| [graft3, 16]kB1 | TRGSFEGYDNGGLPV | 4 | 2 | 1 | 8 |
a
Chloroalkane (ct) and Alexa Fluor 488 (ax) tagged variants were used to study cellular uptake, but only unlabeled peptides are shown here for brevity. Tags were attached via a lysine at position 16.
b
Residue differences to the native peptide are highlighted with underlines. Refer to Figure 1 for the rest of the sequence of native kB1 and Table 1 for the rest of the sequence of the kB1 analogues.
c
Hydrophobic residues: V, P, L, W, F.
d
Negatively charged residues: D, E.
e
Positively charged residues: K, R, H.
f
Polar residues: S, T, Y, N, G.