Table 1.

Kinetic parameters of the wild-type (WT) and C547A recombinant NT5C2.

Kinetic Parameters	WT	C547A
KM Ino (mM)	1.00 ± 0.059	0.83 ± 0.061
KM IMP (mM)	0.10 ± 0.018	0.12 ± 0.022
K50 Mg2+ (mM)	2.00 ± 0.114	0.60 ± 0.101
K50 ATP (mM)	2.00 ± 0.190	1.00 ± 0.087
Ki Pi (mM)	2.00 ± 0.156	2.00 ± 0.218
kcat (sec−1)	52.90 ± 3.35	228.80 ± 16.56

Results are the mean ± SD of three independent experiments. Ino: inosine.