Figure 4.

Silencing suppression activity of V2 mutants substituted with isomorphic amino acids. (A) Co-infiltration of wild-type N. benthamiana leaf patches with pGD-GFP and one of the substitution mutants of V2 including R17K, R43K, R91K, F9Y, Y24F and Y61F. Co-infiltration with pGD-GFP and pGD or pGD-V2 served as negative and positive control, respectively. Photographs were taken at 3 dpa. (B), Western blot analysis of the GFP protein accumulation in agroinfiltrated leaf patches with different constructs at 3 dpa. The Coomassie-blue-stained gel served as a loading control.