Table 2.
Inhibition type, mechanism, and the inhibition constant values of the examined compounds on the diphenolase activity of mushroom tyrosinase.
| Compound | Inhibition Mechanism | Inhibition Type | Ki
1 ± Standard Error (µM) (n = 3) |
Kis
2 ± Standard Error (µM) (n = 3) |
|---|---|---|---|---|
| TSC 1 | reversible | mixed | 5.0 ± 1.4 | 27.5 ± 3.5 |
| TSC 2 | reversible | mixed | 1.0 ± 0.1 | 9.7 ± 3.1 |
| TSC 3 | reversible | competitive | 0.6 ± 0.01 | - |
| TSC 4 | reversible | mixed | 8.0 ± 0.9 | 30.4 ± 0.5 |
| TSC 5 | reversible | mixed | 0.6 ± 0.1 | 3.05 ± 1.1 |
| TSC 6 | reversible | competitive | 0.2 ± 0.06 | 3.5 ± 0.8 |
| TSC 7 | reversible | mixed | 0.7 ± 0.1 | 5.3 ± 2.1 |
| TSC 8 | reversible | mixed | 0.6 ± 0.08 | 5.4 ± 0.1 |
| TSC 9 | reversible | competitive | 0.6 ± 0.06 | - |
| TSC 10 | reversible | mixed | 8.6 ± 0.5 | 43.3 ± 3.0 |
| TSC 11 | reversible | competitive | 0.4 ± 0.02 | - |
| TSC 12 | reversible | mixed | 5.0 ± 1.4 | 27.5 ± 3.5 |
| Kojic acid | reversible | mixed | 26 ± 1 | 72 ± 3 |
1 Inhibition constant for enzyme–inhibitor complex; 2 inhibition constant for substrate–enzyme–inhibitor complex.