Change in IgG and some growth factor constituents within BC during the first few days post-calving. BC was collected at the first and second milking on day 0, and daily for the following 3 days from 6 cows post-calving, i.e., Day 0-1 and 0-2 samples were both collected on the first day after calving. Samples were analysed for IgG and growth factor concentrations using commercial ELISA kits. Immunoreactivity is expressed as % of Day 0-1 sample. EGF (▲), IGF1 (□) and IgG (○). Results are expressed as mean ± SEM of 6 animals per time point, with each sample measured in triplicate. The concentrations of all constituents shown were significantly reduced in each subsequent collection, p < 0.01 vs. their Day 0-1 value. Adapted from [83].