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. 2021 Jan 25;37(3):317–324. doi: 10.1007/s00383-020-04821-x

Fig. 2.

Fig. 2

a Endoscopic biopsy of gastric mucosa from a paediatric patient prior to processing. b, c Characterisation by immunofluorescence of gastric mucosa from endoscopic biopsies. Mucin 6 (MUC6) in green, chromogranin A (CHRA) in red, Mucin 5AC (MUC5AC) in cyan, pepsinogen C (PGC) in magenta, and nuclei in blue (Hoechst). Scale bars 50 μm. d Bright field images of gastric glands isolated from endoscopic biopsies forming epithelial gastric organoids when cultured in chemically defined medium (detailed in methods). Scale bars 100 μm