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. 2021 Jan 25;37(3):317–324. doi: 10.1007/s00383-020-04821-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Bright field images of paediatric gastric organoids in culture. Single cells (top panel) form organoids within 7 days of seeding in Matrigel® (middle & bottom panels). Scale bars 100 μm. b Immunofluorescence panel showing gastric organoids with appropriate polarity and expression of mature cell types. Mucin 5AC (MUC5AC) in cyan, f-actin (F-ACT) in magenta, pepsinogen C (PGC) in green, gastrin (GAST) in red, and nuclei in blue (Hoechst). Scale bars 50 μm