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. 2020 Dec 24;2(1):33–42. doi: 10.1016/j.xfss.2020.12.005

Figure 4.

Figure 4

Expression of CD147 mRNA and protein in human oocytes, embryos, and follicular cells. (A) RT-qPCR analysis of CD147 mRNA in GV- and MII-stage oocytes, BL (n = 3 pools of 6 oocytes or embryos), CC, and GC (n = 3). Expression data were normalized to GAPDH and presented as a bar graph. (B) Capillary Western blot of total CD147 protein (60 kDa) expression and (C) fold change in the CD147 protein abundance in MII-stage oocytes, 1C- and BL-stage embryo, CC, and GC (n = 3 replicates). Arrow indicates the target protein. All bar graphs show the mean ±SEM. Different letters indicate statistical difference (P < .05), determined by 1-way ANOVA followed by Tukey’s test. 1C = 1-cell; ANOVA = analysis of variance; BL = blastocyst; CC = cumulus cells; GC = granulosa cells; GV = germinal vesicle; MII = metaphase II; mRNA = messenger ribonucleic acid; ND = nondetectable; RT-qPCR = quantitative reverse transcription polymerase chain reaction; SEM = standard error of the mean; TMPRSS2 = transmembrane serine protease 2.