Figure 4. Oxidized LDL-mediated regulation of scavenger receptors and efflux transporters mRNA expression during foam cell formation.
(A-D) Mfoam and M0 cells were prepared by incubating WT and Epac1−/− BMDMs with ox-LDL (40 μg/mL) or vehicle, respectively for 48 h. Expression levels of (A) LOX-1, (B) CD36, (C) ABCA1, and (D) ABCG1 were determined by qPCR. Each sample was run at minimum in triplicate for each independent animal (N = 5 or 4 for WT and Epac1−/− respectively). Data are presented as mean ± SEM.