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. 2021 Jan 18;8(1):12. doi: 10.3390/vetsci8010012

Figure 2.

Figure 2

Receiver operating characteristic (ROC) analyses of the triplex SBV–AKAV–SHUV ELISA using 477 negative sera and 238 SBV antibody-positive sera, 36 sera displaying antibodies against AKAV and 53 SHUV seropositive samples. For the SBV assay (a,d), the ROC curves are shown separately for cattle (blue), sheep (black), goat (green) and wild and zoo animals (grey), the combined ROC curve that includes all species is depicted in red. The ROC analyses were performed using two different set-ups, for the first (a–c) only sera negative against all three simbuviruses were included as negative samples, while in the second approach (d–f) negative samples additionally include sera negative against the given antigen, but positive towards another Simbu serogroup virus. SBV, Schmallenberg virus; AKAV, Akabane virus; SHUV, Shuni virus.