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. 2021 Jan 18;8(1):12. doi: 10.3390/vetsci8010012

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Reproducibility and repeatability of the SBV-AKAV-SHUV ELISA. A negative cattle and a negative sheep serum as well as sera antibody-positive towards SBV, AKAV or SHUV were tested against the SBV (b), AKAV (c) and SHUV (d) antigens in five replicates each in five independent approaches. The boxplots represent the results of all 25 replicates. The cut-off values are indicated by horizontal dashed lines. (a) Adsorbance values of the negative and positive controls that were included in every approach, the boxplots represent the results of all 10 replicates.