Table 2.

Diagnostic characteristics of the triplex SBV-AKAV-SHUV ELISA. The first row per antigen and, if applicable, animal species includes values calculated for samples positive against the given antigen and negative against all simbuviruses. In the second row, negative samples additionally include sera negative against the given antigen, but positive for another Simbu serogroup virus. AUC: area under the curve, CI: 95% confidence interval.

Antigen	Animal Species	AUC	Sensitivity	Specificity
SBV	cattle-1	0.9519 (CI: 0.9259 to 0.9779)	92.11% (CI: 83.60% to 97.05%)	89.58% (CI: 84.37% to 93.52%)
	cattle-2	0.9418 (CI: 0.9149 to 0.9686)	92.11% (CI: 83.60% to 97.05%)	87.98% (CI: 83.10% to 91.86%)
	sheep-1	0.9566 (CI: 0.9326 to 0.9806)	93.42% (CI: 85.31% to 97.83%)	86.79% (CI: 80.52% to 91.63%)
	sheep-2	0.9532 (CI: 0.9289 to 0.9775)	93.42% (CI: 85.31% to 97.83%)	84.80% (CI: 78.52% to 89.82%)
	goat-1	0.9186 (CI: 0.8553 to 0.9818)	94.59% (CI: 81.81% to 99.34%)	76.92% (CI: 66.00% to 85.71%)
	goat-2	0.9196 (CI: 0.8571 to 0.9821)	94.59% (CI: 81.81% to 99.34%)	77.22% (CI: 66.40% to 85.90%)
	wild/zoo animals-1	0.8835 (CI: 0.8199 to 0.9471)	73.47% (CI: 58.92% to 85.05%)	79.17% (CI: 65.01% to 89.53%)
	wild/zoo animals-2	0.8835 (CI: 0.8199 to 0.9471)	73.47% (CI: 58.92% to 85.05%)	79.17% (CI: 65.01% to 89.53%)
	overall-1	0.9339 (CI: 0.9159 to 0.9520)	89.08% (CI: 84.40% to 92.74%)	85.53% (CI: 82.05% to 88.57%)
	overall-2	0.9292 (CI: 0.9110 to 0.9474)	89.08% (CI: 84.40% to 92.74%)	84.56% (CI: 81.20% to 87.53%)
AKAV	overall-1	0.9536 (CI: 0.9247 to 0.9825)	69.44% (CI: 51.89% to 83.65%)	97.06% (CI: 95.12% to 98.39%)
	overall-2	0.9386 (CI: 0.9079 to 0.9692)	69.44% (CI: 51.89% to 83.65%)	94.68% (CI: 92.80% to 96.19%)
SHUV	overall-1	0.9384 (CI: 0.8997 to 0.9771)	84.91% (CI: 72.41% to 93.25%)	93.29% (CI: 90.66% to 95.37%)
	overall-2	0.9234 (CI: 0.8792 to 0.9676)	84.91% (CI: 72.41% to 93.25%)	89.39% (CI: 86.89% to 91.55%)